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[Abstract]2021 Sep 23;12(10):868

The flow-through fractions were pooled and concentrated by centrifugation with a 10 kDa cutoff and subjected to size exclusion chromatography on a Superdex-200 column in Buffer C (25 mM Tris-HCl, pH 8.0, 500 mM NaCl, 2 mM EDTA) and monomeric fractions pooled