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Long-chain fatty acids require an intracellular taxi to enter the mitochondria where they can be oxidized (burned) for energy (ATP) production

However, AKT phosphorylation at Thr308 was not detectable in HCT116 cells (data not shown)

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However, unlike the excluded volume effect of crowding agents, the confinement effect of vesicles can be studied separately from the chemical interactions by experimental comparison with non-encapsulated RNA that is exposed to vesicles (RNA outside vesicles)