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doi: 10.1096/fj.201601222R 186 ShahV

The amplified products were recombined and transformed on ice using DH5 competent cells, based on which the sequence for the plasmid was extracted and confirmed

therefore, fatty acids of both classes are considered essential nutrients and must be derived from the diet (For more information, see the article on Essential Fatty Acids) (30)

Celastrol impedes degradation of the merlin protein in malignant meningioma cells carrying a missense mutation in the NF2 gene at ~1M (77) ( Table 1 )