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Maintaining improvements long-term requires either continued use or transition to lifestyle interventions that sustain AMPK activation (resistance training, caloric cycling)

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All treatments of mice in this study were in strict agreement with guidelines on ARRIVE and recommendations from an NIH-sponsored workshop regarding experimental design and reporting standards (41)

PKR promotes intestinal epithelial cell homeostasis and survival by activating the eIF2-mediated UPR, as well as the STAT3 and AKT signaling pathways