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Under normoxic conditions HIF1 is hydroxylated and subsequently ubiquitinated for proteasomal degradation, whereas under hypoxic conditions its hydroxylation is blocked leading to its accumulation, dimerization with its beta subunit and subsequent translocation into the nucleus, where it regulates the expression of genes linked to cellular transformation, cell proliferation and angiogenesis [154156]

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Determination of 20S proteasome levels in biological samples (cell lysates, tissue extracts, plasma, serum) Comparison of 20S proteasome levels in plasma/serum samples associated with a particular disease/illness with samples from healthy controls Investigation of variation in 20S proteasome levels in abnormal cell lines/tissues This kit provides the means to quantify 20S proteasome concentrations in biological samples using a sandwich ELISA technique, utilizing two 20S proteasome specific antibodies for capture and detection purposes together with a highly sensitive substrate