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The shortening fraction was determined by dividing the difference of the heart chamber width at the end of the diastolic phase and the end of the systolic phase with the width of the heart chamber at the end of the diastolic phase (Figure 3AC)

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Third, translational gaps between preclinical models and clinical practice are prominent: most therapeutic candidates (e.g., natural products and repurposed drugs) have only been validated in rodent models, with limited data on human renal tissue responses, optimal dosing windows, or potential drugdrug interactions in clinical settings (e.g., in patients undergoing kidney transplantation or cardiovascular surgery)

It prolonged the damage caused by drinking too much, and it was also extremely painful for the addicted person

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