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With proper care, the results can last for several months

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To relieve this intrinsic autoinhibition and stabilise a productive binding interface for its substrates, two critical phosphorylation events are essential: autophosphorylation at serine 281 (S281) within the activation segment and transphosphorylation at threonine 444 (T444) within the C-terminal hydrophobic motif by upstream kinases [41,42,43,44]

Wei T, Chen C, Hou J, Xin W, Mori A

CRISPR/Cas9-based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering favors DNA repair via non-homologous end joining, the newly inserted gene may have errors introduced to the nucleotide sequence during the DNA repair process