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As determined by Giemsa staining and by having an experienced technician count infected cells under the light microscope, the parasitemia for the 3D7 strain was 3.90.5% in the GR-deficient cells and 4.00.4% in the controls

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Supplementation with GSH, at a concentration within the range found in the TIF, or with cysteinylglycine rescued cancer cell growth in cystine-free conditions (Fig

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10.1007/s12291-017-0667-0 109

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An aliquot of 20 l of the third-round virus mixture was used to infect 500 ml (SFM900-II medium with 5% FBS, Thermo Fisher) of Sf9 cells (1.5 10 6 /ml) for protein expression in an orbital shaker (Shanghai Zhichu Instrument) at 27 C and 200 rpm

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