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2016;6:e0098 Hodges RE et al

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There are two main limitations of the redox array: firstly, a positive signal on the array could also be due to a biotinylated protein that binds to the antibody specific ligand thus resulting in a false positive signal

NADPH oxidases generate ROS as O 2 or H 2 O 2 depending on the isoform and contextNOX1/2/3 primarily release O 2 that dismutates to H 2 O 2 , NOX4 predominantly yields H 2 O 2 and Ca-activated NOX5 together with epithelial DUOX1/2 contribute directly to H 2 O 2 production at barrier surfaces (Kim and Moon 2024)