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Inside a three-necked round-bottom flask equipped with a condenser, magnetic stirrer, gas inlet/outlet, DMF (20 mL) and isopropanol (1:1), and MeO-PEG-Br macroinitiator (0.1 mmol, 0.5 g) was also added and stirred 1 h to provide the macroinitiator dispersion

Free Radic Biol Med 179:109118 Shao C, Yuan J, Liu Y et al (2020) Epileptic brain fluorescent imaging reveals apigenin can relieve the myeloperoxidase-mediated oxidative stress and inhibit ferroptosis [J]

ISH probes were prepared by in vitro transcription with DIG RNA Labeling Mix (Roche Applied Science, #11277073910) or Fluorescein RNA Labeling mix (Roche Applied Science, #11685619910) and T7 polymerase (Promega, #P2075), T3 polymerase (Roche Applied Science, #11031163001) or SP6 polymerase (Roche Applied Science, #10810274001)

Some researchers find that injecting in the evening before sleep allows them to sleep through the worst of any initial nausea

We used a portable personal breathalyzer (BA Track S80 model) to determine BAC