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Diets lacking in complementary nutrients necessary for B12 absorption may lead to less effective results and a subsequent feeling of being worse post-injection

This decline leads to insufficient activation of sirtuins (SIRT1SIRT7)NAD+-dependent deacetylases that regulate mitochondrial biogenesis via PGC-1, DNA repair, and cellular stress resistance

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The rationale traces back further, to Kraus and colleagues in 2014 ( Nature ), who knocked down NNMT genetically in mice and protected them from diet-induced obesity