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StAR, steroidogenic acute regulatory protein, and CYP11A1, cytochrome P450 family 11 subfamily A member 1, are markers for Leydig cells, while 3-HSD, 3-beta-hydroxysteroid dehydrogenase, is a key enzyme in testosterone synthesis

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Three structurally different classes of compounds considered to be of particular interest as starting points for the development of small-molecule IRAP inhibitors were identified

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The freeze-thaw cycle can cause protein aggregation and denaturation, reducing potency