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After a 12-h culture process, the cells that adhered to the underside of the chamber for 30 min were fixed with 4% paraformaldehyde, stained with crystal violet for 15 min, and later got calculated under an optical microscope (Zeiss, Germany)

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doi: 10.1111/dom.14734

Bremelanotide has no food interaction on efficacy (though we avoid heavy food to reduce nausea) and a different side effect profile (no visual disturbances or risk of low blood pressure