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Finally, CAPDH and Histone H3 act as loading controls 39 , the relative protein expression was standardized to GAPDH and Histone H3 from the same sample and quantified using a AlphaEase FC image analyzer software (Alpha Innotech, California, USA)

163,164 However, ineffective clearance of apoptotic neutrophils results in necrosis and the subsequent release of toxic granulocytes and DAMPs into the extracellular space, exacerbating the local inflammatory response and tissue damage

As a result, bacterial antigens may enter sacroiliac joints and the spine through lymph nodes, inducing inflammatory responses (66)