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This remarkable selectivity stems from 5-amino-1MQs specific interaction with the nicotinamide-binding pocket of NNMT, rather than the SAM-binding site common to many methyltransferases

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For the SILAC experiments, we constructed an arg4 lys2 strain, and seeded cells from the same colony in synthetic medium with cold (R0K0) or hot (R6K4) amino acid isotopes