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Sorafenib induces pigmentation via the regulation of beta-catenin signalling pathway in melanoma cells

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Cleared lysate was loaded on a 5 ml StrepTactin column (IBA) followed by the first wash using 10 ml of H buffer (20 mM HEPES pH 7.5, 5% glycerol, 0.01% NP40, 1 mM -mercaptoethanol) containing 500 mM NaCl and 1 mM ATP, and the second wash using 25 mL of H buffer containing 150 mM NaCl

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