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"Finding that interesting loop later led to our understanding of the sensing mechanism," Birsoy says

However, in clinical practice, it is important to carefully evaluate and individually manage patients who require PPI therapy to avoid long-term abuse

Post-infection, virus was removed and cells were selected with puromycin (lentiCRISPR-v2), blasticidin (pMXS_IRES_Blast) or by flow sorting of top 1% GFP+ cells (lentiCRISPR-v1(GFP))