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It is a tripeptide with a gamma peptide linkage between the carboxyl group of the glutamate side chain and the amine group of cysteine, and the carboxyl group of cysteine is attached by normal peptide linkage to a glycine

Templeman JR, Thornton E, Cargo-Froom C, Squires EJ, Swanson KS, Shoveller AK

For double labelling, Alexa Fluor 568 streptavidin conjugate (Invitrogen, 1:250) was used for visualising vascular staining (IB4), donkey anti-mouse (Alexa Fluor 488 conjugate, Invitrogen, 1:250) and donkey anti-rabbit Alexa Fluor 488 conjugate (Invitrogen) diluted in 1% normal horse serum in PBS was used for visualising immunostatining of IGF-1 receptors

Depending on results and providers discretion the injections may be repeated up to 3-4 times per year for sustained pain relief

The investigators informed patients or their surrogates concerning the study orally and written informed consent was given before entry into the study