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In another study, nerve ligation increases oxidative stress, documented by increased levels of MDA, increased oxidation state of proteins [43]

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Subsequently, all unique sequences were aligned against the SILVA v138.1 reference alignment from the mothur repository ( Taxonomic classification was assigned to amplicon sequence variants (ASVs), retaining only those attributed to archaeal and bacterial domains

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For comparisons of PNM concentrations, all groups were compared with each other using the same approach