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Cyanocobalamin is the most used and given in doses from 100 to 1,000 mcg

(1997) Vitamin B12 treatment for delayed sleep phase syndrome: a multi-center double-blind study

1 This is the primary form of folate found in blood and is necessary for the multistep process that converts the amino acid homocysteine to methionine, an amino acid that our bodies use to make proteins

For NMR analysis, polar fractions were resuspended in 630 l of PBS (pH 7.4), adding 70 l of 2 H 2 O solution (containing 1 mM sodium 3-trimethylsilyl [2,2,3,3-2H4]propionate (TSP) as a chemical shift reference for 1 H spectra) to provide a field frequency lock, reaching 700 l of total volume