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If small particles remain, continue gentle swirling for another 30-60 seconds

10.1186/bcr1275 [DOI] [PMC free article] [PubMed] [Google Scholar] Dixit V, Chaubey KK, Dayal D, Chole PB, Manjunath BT, Bachheti RK, Gupta PC, Bachheti A, Worku LA (2025) Natural products in aging: cellular mechanisms and emerging therapeutics for age-related disorders

It's also popular for anti-aging, as it protects cells and mitochondria from oxidative damage, which is a key factor in the aging process

Cells were lysed at 4C in icecold radioimmunoprecipitation assay lysis buffer (Millipore, Billerica, MA, USA) or 3[(3cholamidopropyl)dimethylammonio]1propane sulphonate lysis buffer (Cell Signaling Technology) as indicated in figure legends

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