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Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)

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While outside the purpose and scope of this review, it is important to note that alongside nutrient synergy exists the concept of nutrient antagonism (110, 118)

Alleles associated with morphea are in parallel strongly associated with rheumatoid arthritis (RA), autoimmune thyroid disease (AITD), multiple sclerosis (MS) and type 1 diabetes mellitus