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Description

Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

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If you have reconstituted with sterile water by mistake and cannot use the entire vial within 24 hours, the safest approach is to discard it and start fresh with bacteriostatic water