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Aside from this, they are also involved in the catalysis of fatty acid reduction and the metabolism of phospholipids and DNA hydroperoxidases, which are all products of oxidative stress [6]

Step 8: Label the vial immediately

For the generation of triple mutants, the kanamycin cassette was removed from the gshA, gsiC or gshA, gsiB double deletion mutant using the plasmid 709-FLPe as indicated by the supplier (Gene Bridges, Heidelberg, Germany)