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Western blot analysis One microgram of SYN was added to denaturing sample buffer (62.5 mM Tris, pH 6.8, 10 % (v/v) glycerol, 2 % (w/v) SDS, 5 % (v/v) -mercaptoethanol, and 1 % (w/v) bromophenol blue), boiled for 90 s and subjected to polyacrylamide gradient (416 %) gel electrophoresis under denaturing conditions followed by transfer to polyvinylidene difluoride (PVDF) membranes (PerkinElmer, Waltham, MA)

A review summarized the evidence indicating that SOD mimetics have a significant efficacy in several animal models that mimic oxidative stress injuries ( 2 dismutation activity and some of them, such as Mn(III) meso-tetrakis (N- methylpyridinium-2-yl)porphyrin (MnTM2-pYp5 + ) and Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-pYp5 + ), demonstrated a powerful SOD activity ( + and also Mn(III) meso-tetrakis (di-N-ethylimidazole) porphyrin (MnTDE-2-ImP5 + ) have been shown in animal models of inflammation-associated conditions such as breast tumor (Rabbani et al., 2009) and cardiovascular system damage ( + was found to amend oxidative stress-induced inflammation and enhance insulin sensitivity and glucose tolerance in a high-fat diet-induced mouse model of type 2 diabetes (Coudriet et al., 2017)

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Yez JA, Chemuturi NV, Womble SW, Sayre CL, Davies NM

Activation of Type 1 CRH receptor isoforms induces serotonin release from human carcinoid BON-1N cells: an enterochromaffin cell model