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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

We excluded animals that could not be located on the plate from statistical analysis

Because collagen is a protein-rich supplement and tied to amino acids involved in creatine metabolism, questions like Does collagen increase creatinine levels matter to anyone monitoring renal health.

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For example, a study found that Bifidobacterium longum NCC3001 normalizes anxiety-like behavior and hippocampal brain-derived neurotrophic factor (BDNF) in mice with infectious colitis