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To evaluate spermatogenesis and male fertility in mice, Computer-Aided Sperm Motion Analysis (CASA) was conducted on epididymal sperm to determine motility parameters, including the percentage of motile and forward progressive sperm

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To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig

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