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Brains were collected and fixed in 4% paraformaldehyde for 24 h, dehydrated, frozen with OCT, and cut into 20 m frozen slices by using a freezing microtome (Leica, Germany)

In contrast, in arginine medium both the gdh3 / gdh3 and wild-type strains completely shifted from yeast to hyphal growth

In some cases, patterns of side effects across multiple subjects may need protocol changes or more safety measures

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Solvent Accessible Surface Area (SASA) SASA analysis captures the surface area of the protein which is available to the solvent where a higher SASA value represents a relative expansion and vice versa