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The activation of EMT is considered a critical event in the early stages of tumour metastasis ( In addition, AS-IV (5, 10, 20 M) can inhibit the proliferation, migration and invasion of Huh-7 cells (Zhou, 2022) investigated the core targets and key signaling pathways of AS-IV in HCC

ROS are byproducts of aerobic metabolism and primarily include superoxide anion (O 2 ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH)

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After that, the cells were washed with PBS for three times and incubated with 100 L serum-free medium containing 10 L CCK-8 for 1 h

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