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Description

PDT has been studied since the 1980s (268, 269), and has shown promising efficacy in many glioma preclinical studies (270272)

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Without its signal sequence, AP accumulates in the cytoplasm, and only a tiny fraction is exported to the periplasm (Eben and Imlay, 2023)

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Resuspend the cell pellet in Solution B (for the four-step gradient) OR the 21.6% (w/v) iodixanol solution (for the two-step gradient)

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Replacing animal protein with plant protein significantly reduces MASLD risk [105,128]

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Afterwards, viability, cell surface antigen and intracellular cytokine staining was performed as described above

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