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Each puncture through a rubber stopper dulls the needle tip and introduces microscopic rubber particles into your solutionthese particles can trigger immune responses in research models and create confounding variables in your data

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These inflammatory macrophage formations can result in associated microscopic muscle necrosis

For example, with reference to the clinical ocular trials mentioned in Sosne and Kleinman, the researchers noted that no long-term safety studies have been done with T4 treatment in the eye for more than a month [12]