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Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets

[DOI] [PMC free article] [PubMed] [Google Scholar] 86.Moorthy D, Merrill R, Namaste S, Iannotti L

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Regardless of the solution used, freezing a reconstituted peptide is generally discouraged, as it can degrade the compounds structure