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Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism

Since N-terminal sequences of AsGGT1, AsGGT2, and AsGGT3 are long and may contain the signal sequence for secretion or targeting to cellular organelles, three types of fusion proteins, GFP C-terminally fused to the N-terminal 100-amino acid residues of GGT (AsGGT1 N100 -GFP, AsGGT2 N100 -GFP, and AsGGT3 N100 -GFP), GFP C-terminally fused to the N-terminal 300-amino acid residues of GGT (AsGGT1 N300 -GFP, AsGGT2 N300 -GFP, and AsGGT3 N300 -GFP), and GFP C-terminally fused to the full-length GGT protein (AsGGT1 Full -GFP, AsGGT2 Full -GFP, and AsGGT3 Full -GFP), were analyzed

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