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Melatonin further reinforces insulin sensitivity by synchronizing circadian insulin secretion, improving mitochondrial efficiency in hepatocytes and skeletal muscle, and attenuating the nocturnal cortisol overshoot typical in PCOS (226, 227)

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Cell viability and cell-free tyrosinase inhibition assay Considering the potential of selected flavonoids as mh-Tyr inhibitors and so as an active ingredient for the formulation against hyperpigmentation, evaluation of these compounds for their cell viability efficacy in mammalian cell lines is required before furthering the experimental analysis