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As we specifically observed impaired GPX4 activity in IECs from the inflamed (but not uninflamed) mucosa of CD patients, it appears plausible that GPX4 deficiency is no primary defect in CD but occurs secondary to insults (likely caused by yet unidentified cellular stressors) in the inflamed mucosa

Some researchers wear disposable nitrile gloves, which is a good practice but not strictly required if your hands are freshly washed

The cell pellet was resuspended and lysed in lysis buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 1% Triton-X100 (v/v), 2 mM MgCl 2 , 0.5 mM TCEP) with protease inhibitors (1 mM PMSF, 1 M pepstatin, 4 M leupeptin, 0.3 M aprotinin) for 30 min at 4 C

doi: 10.1158/1078-0432.CCR-15-3153 60 LeeEQKuhnJLambornKRAbreyLDeAngelisLMLiebermanFet al

Forskolin, an Adenylcyclase/cAMP/CREB Signaling Activator Restoring Myelin-Associated Oligodendrocyte Destruction in Experimental Ethidium Bromide Model of Multiple Sclerosis