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In rats, administration of chloroquine (an inhibitor of intralysosomal proteolysis) causes transient accumulation of phosphorylated tau in neuronal lipofuscin granules, particularly in regions such as the amygdala, hippocampus and entorhinal cortex [124]

The membrane was blocked with 5% skim milk in TBST for 1 h and was then incubated with the primary antibody for 1 h at room temperature (RT) or overnight at 4 C

Therefore, many studies are focused on developing novel non-dopaminergic drugs that can prevent or even reverse the degeneration of DA neurons

The antioxidant component tocopherols also aids in protection against the detrimental effects of free radicals and mitigates heart-related problems (26)

I would like to thank Irina Minich for assistance in the manuscript preparation