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18 The beneficial effects of classical antihypertensive agents, such as -adrenergic blockers, angiotensin I converting enzyme (ACE) inhibitors, AT-II receptor antagonists, and calcium channel blockers, may be mediated, in part, by decreasing vascular oxidative stress

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Subsequently proteins were transferred onto a nitrocellulose membrane (BioTraceNT, Pall Life Sciences, Dreieich, Germany) by tank blotting (transfer buffer: 20 mM Tris/HCl, pH 8.7, 150 mM glycine and 20% (v/v) methanol)