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Burns 41:17961804 Fowler M, Clifford JL, Garza TH, Slater TM, Arizpe HM, Novak J, Petz LN, Loyd DR (2014) A rat model of full thickness thermal injury characterized by thermal hyperalgesia, mechanical allodynia, pronociceptive peptide release and tramadol analgesia

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PBMCs were collected from the interface between the plasma and Histopaque-1077, while PMNs were obtained from the interface between the Histopaque-1077 and Histopaque-1119

Another study by the same authors used a similar infusion but this time with three higher intensity 45 s sprints with a fourth sprint at 130% peak oxygen consumption[26]

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