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The level of AGEs in the urine possibly indicates that in vivo AGE levels are positively correlated with dietary glycemic load (Maasen et al

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Cells were fixed with 4% PFA for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 5 min and blocked with blocking buffer (10% normal goat serum in PBS) for 30 min

[DOI] [PMC free article] [PubMed] [Google Scholar] 193.Zhang J., Yao M., Xia S., Zeng F., Liu Q

The disability rate increases dramatically with the course of the disease, reaching 70% at 3 years in untreated patients, and the systemic complications reduce life expectancy by 1015 years (2)