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But the second step in Class II involves a nucleophilic attack by the 3'-OH of the A76 ribose on the carboxyl carbon of the aminoacyl adenylate intermediate, forming aminoacyl-tRNA and AMP

Beyond these mechanisms, TXNIP demonstrates mitochondrial translocation in hyperuricemic inflammation, where its interaction with NLRP3 induces inflammasome assembly and subsequent pro-inflammatory cytokine secretion (53)