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SEC-MALS analysis of GUS enzymes Rg GUS, Lr GUS, Fp GUS, and Bd GUS were analysed on a Superdex 200 size exclusion column connected to an Agilent FPLC system, Wyatt DAWN HELEOS II multi-angle light scattering instrument and a Trex refractometer

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Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)

The authors defined COVID-19 disease severity as either moderate or mild using metrics from the National Early Warning Score methodology, where respiratory rate, oxygen saturation, oxygen supplement, body temperature, systolic blood pressure, heart rate, and consciousness were used as evaluating criteria 49,96