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Cloudy or gel-like solution: treat it as failed, not ready for accurate research

After removing the supernatant, the lymphocyte pellet was resuspended in 100200 L saline

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Vascular elements on hematoxylin and eosin stained slides as well as immunohistocemically positive elements were examined in a blinded fashion using hot-spot assessment and ISSA program (VAMSTEC, Zagreb, Croatia)

Importantly, anti-VEGF, but not anti-VEGFR-2, antibody considerably augmented infiltration of injected cells into the tumor, suggesting that normalization of tumor vasculature by suppressing VEGF/VEGFR-2 axis could upsurge extravasation of administrated T cells into the tumor [142]