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Human T-cells were isolated from healthy donor PBMCs using the EasySep Human T-cell Isolation Kit, cultured in ImmunoCult-XF T-cell Expansion Medium, and stimulated to grow with ImmunoCult Human CD3/CD28/CD2 T Cell Activator supplemented with 100 U/mL IL-2 (all from StemCell Technologies)

Immunomodulatory Effects Black cumin and TQ were shown to exert immunostimulatory functions as reported in several preclinical and clinical studies

Obesity was defined as presenting a body mass index (BMI) over two standard deviations above the mean of the age- and sex-adjusted reference population [12], and the OGTT was used to diagnose IR according to standardized criteria [13]

Ecotoxicology 22, 308318

[DOI] [PMC free article] [PubMed] [Google Scholar] 247.Takamura K., Nasuhara Y., Kobayashi M., Betsuyaku T., Tanino Y., Kinoshita I., Yamaguchi E., Matsukura S., Schleimer R.P., Nishimura M