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They were isolated from the sciatic nerves of adult male Wistar rats purchased from Pasteur Institute, Tehran, Iran according to Terraf et al

More so, as stated in this 2019 study, "there is no established upper limit for vitamin B12 intake, as it has a low potential for toxicity." Although taking extremely high dosages of vitamin B12 is deemed safe, it is still not recommended to go above and beyond recommended vitamin B12 intake levels as mentioned above

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Endotoxin control is critical for minimizing biological interference and maintaining integrity in sensitive analytical systems