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After a final wash step, a tetramethylbenzidine (TMB) peroxidase substrate solution (KPL Inc.) was added to each well and absorbance of the developed color was measured by a colorimetric plate reader (Molecular Device)

Short-term and long-term effects of transient exogenous cortisol manipulation on oxidative stress in juvenile brown trout

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energy (ATP) is used to transport substances out of the cell against a concentration gradient in multiple different organs including the brain during this phase (Dean et al., 2001

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