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Increased intracellular ROS can induce apoptosis by various mechanisms, including endoplasmic reticulum (ER) stress, mitochondrial impairment, and activation of death receptors (Liu et al., 2021)

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After incubation of hemolyzed total blood with DTT, we observed a conjugation rate of GSH with CDNB higher than that found in the absence of reducing treatment, suggesting the presence of a significant, not spurious amount of oxidized e-GST (Figure 2a)