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Young et al., 2009

After centrifugation, the homogenate was incubated with 200ml of KPBS pre-washed anti-HA magnetic beads (Thermo Scientific Pierce 88837) on a rotator shaker for 5min at 4C

Additionally, ferroptosis-derived ROS can activate the AMPK/ULK1 pathway to initiate autophagy (67), although the precise molecular cascade in LC cells is not fully elucidated