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Cell homogenates were then centrifuged for 30 min at 10,000 rpm at 4 C and the supernatants obtained were immediately used for the estimation of antioxidant status and lipid peroxidation

After washing, the NP solution was frozen down at 20C for 4 h and then put in a lyophilizer for 48 h

Lentivirus infection was carried out in 6-well plates by mixing 500 l virus supernatant and 500 l regular culture medium containing 0.8 l polybrene (10 mg/ml)

Emerging regulatory paradigms in glutathione metabolism

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