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Cell culture and viability assay Raw 264.7 cells, originating from mouse macrophages, were maintained in a complete medium consisting of 90% high-glucose Dulbeccos Modified Eagle Medium (DMEM) and 10% fetal bovine serum (FBS), at 37 C in a 5% CO 2 incubator

the peptide comprises a peptide comprising or consisting of 8 or more contiguous amino acids from the amino acid sequence of any one of SEQ ID NOs 1-75

Avoid strenuous activity for 24-48 hours, but walking is not considered strenuous

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Third, and perhaps most relevant, it promotes fibroblast migration and collagen synthesis , the exact cellular processes needed to rebuild the damaged annulus fibrosus