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Lu99 cells in 3D culture were incubated in 24-well culture plates for 24 h, each supernatant was then collected, and 500 l of each supernatant was stratified in tube-induction medium to induce tube formation in sandwich culture described above

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In the myb4 hy5 double mutant, CHS expression was similar to the hy5 mutant, indicating that HY5 acts downstream of MYB4 in regulating CHS expression under control condition ( Figures 6E, F )

doi:10.1097/MAO.0b013e3180686e4a

Born, J